Starting the CO2 Cultures
We've finally done it!
Team Bravo 134 has started the big experiment: the CO2 exposure experiment is underway in the Phase III aquarium of Crary Labs. Yesterday morning started with the spawning of 36 female urchins to see if sufficient eggs could be collected. Dr. Sewell is shown below starting the process.
Below are our fabulous females! Twenty of them provided eggs of outstanding quality; a mix of these were used to start the cultures.
Emily was in charge of mixing the eggs just before the fertilization step - she wields a mean pipette.
Once fertilized, the embryos were immediately distributed into the culture buckets in the CO2 system. We are now 24 hours into the experiment and thus far the embryos seem to be developing. We will be watching the cultures for the next 48h and will update the blog in a few days. Cross 'em, if you got 'em!